Essential Genetics 6th Edition by Daniel Hartl

Exercise 7

A 3.1-kilobase linear fragment of DNA was digested with Pst I and produced a 2.0 kb fragment and a 1.1-kb fragment. When the same 3.1-kb fragment was cut with Hind III, it yielded a 1.5-kb fragment, a 1.3-kb fragment, and a 0.3-kb fragment. When the 3.1-kb molecule was cut with a mixture of the two enzymes, fragments of 1.5, 0.8, 0.5, and 0.3 kb resulted. Draw a map of the original 3.1-kb fragment, and label the restriction sites and the distances between these sites.