Essential Genetics 6th Edition by Daniel Hartl

Exercise 3

A double mutant produced by recombination contains two single nucleotide frameshifts separated by about 20 base pairs. The first is an insertion, the second a deletion. The amino acid sequences of the wildtype and mutant polypeptide in this part of the protein are as follows:

What are the original and the double-mutant mRNA sequences? Which nucleotide in the wildtype sequence is the frameshift addition? Which nucleotide in the double-mutant sequence is the frameshift deletion? (In working this problem, you will find it convenient to use the conventional symbols Y for unknown pyrimidine, R for unknown purine, N for unknown nucleotide, and H for A or C or T.)