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Essential Genetics 6th Edition by Daniel Hartl
Edition 6ISBN: 978-1449686482Essential Genetics 6th Edition by Daniel Hartl
Edition 6ISBN: 978-1449686482 Exercise 21
After doing a restriction digest with the enzyme Sse I, which has the recognition site
(the arrow indicates the position of the cleavage), you wish to separate the fragments in an agarose gel. In order to choose the proper concentration of agarose, you need to know the expected size of the fragments. Assuming equivalent amounts of each of the four nucleotides in the target DNA, what average fragment size would you expect?
(the arrow indicates the position of the cleavage), you wish to separate the fragments in an agarose gel. In order to choose the proper concentration of agarose, you need to know the expected size of the fragments. Assuming equivalent amounts of each of the four nucleotides in the target DNA, what average fragment size would you expect?Explanation
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The enzyme SseI digests at the site
....
Essential Genetics 6th Edition by Daniel Hartl
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