Chat with AI
Essentials of Genetics 9th Edition by Michael Palladino William Klug, Michael Cummings, Charlotte Spencer
Edition 9ISBN: 978-1292108865Essentials of Genetics 9th Edition by Michael Palladino William Klug, Michael Cummings, Charlotte Spencer
Edition 9ISBN: 978-1292108865 Exercise 2
Review the chapter concepts list on p.1. Most of these concepts are related to the discovery of DNA as the genetic material and the subsequent development of recombinant DNA technology. Write a brief essay that discusses the impact of recombinant DNA technology on genetics as we perceive the discipline today.
Explanation
Verified
Verified
"Genetic engineering" is based on the recombinant DNA (deoxyribonucleic acid) technology, the modification of an organism's genetic makeup, which results in the modification of its phenotype. A selected gene is modified and reinserted into an organism of the same species or a gene from an individual of one species is inserted into another species, now the resulting one is a "transgenic organism" in either of the cases. The most commonly used gene transfer techniques are microinjection, liposome fusion, electroporation, or else using chemicals like polyethylene-glycol.
The experiment to test whether GMO (genetically modified organism) has gene of interest, first collect the DNA from organism. Then conduct the RFLP (restriction length polymorphism) or STR (simple tandem repeats) analysis or other advanced molecular biology techniques. Compare the results of Unknown DNA with rest of wild organisms. Then sequence the DNA, which shows similarity, from this data use NJ (neighbor joining) method or other tree construction methods by using bioinformatics techniques.
Recombinant gene technology is now a day's being used to increase the yielding of certain plant and microbial products and also in the synthesis of drugs. For example, human insulin (humulin) is produced by the E.coli by inserting the insulin gene into the bacteria by recombinant technology. This is really very useful in the treatment of diabetes, millions of people overall the world is now using this product. One disadvantage of rDNA (recombinant DNA) drugs from the biological point of view is, some people may be sensitive towards these products because of antigenicity.
The experiment to test whether GMO (genetically modified organism) has gene of interest, first collect the DNA from organism. Then conduct the RFLP (restriction length polymorphism) or STR (simple tandem repeats) analysis or other advanced molecular biology techniques. Compare the results of Unknown DNA with rest of wild organisms. Then sequence the DNA, which shows similarity, from this data use NJ (neighbor joining) method or other tree construction methods by using bioinformatics techniques.
Recombinant gene technology is now a day's being used to increase the yielding of certain plant and microbial products and also in the synthesis of drugs. For example, human insulin (humulin) is produced by the E.coli by inserting the insulin gene into the bacteria by recombinant technology. This is really very useful in the treatment of diabetes, millions of people overall the world is now using this product. One disadvantage of rDNA (recombinant DNA) drugs from the biological point of view is, some people may be sensitive towards these products because of antigenicity.
Essentials of Genetics 9th Edition by Michael Palladino William Klug, Michael Cummings, Charlotte Spencer
Why don’t you like this exercise?
Other Minimum 8 character and maximum 255 character
Character 255
