Essentials of Genetics 9th Edition by Michael Palladino William Klug, Michael Cummings, Charlotte Spencer

Exercise 20

Several temperature-sensitive mutant strains of E. coli display the following characteristics. Predict what enzyme or function is being affected by each mutation.
(a) Newly synthesized DNA contains many mismatched base pairs.
(b) Okazaki fragments accumulate, and DNA synthesis is never completed.
(c) No initiation occurs.
(d) Synthesis is very slow.
(e) Supercoiled strands remain after replication, which is never completed.