Essentials of Genetics 9th Edition by Michael Palladino William Klug, Michael Cummings, Charlotte Spencer

Exercise 6

Using DNA sequencing on a cloned DNA segment, you recover the nucleotide sequence shown below. Does this segment contain a palindromic recognition sequence for a restriction enzyme? If so, what is the double-stranded sequence of the palindrome, and what enzyme would cut at this sequence? (Consult Figure for a list of restriction sites.)5?-GTCATAGGATCCGTA-3?
FIGURE Common restriction enzymes with their restriction sites, DNA cutting patterns, and sources. Arrows indicate the location in the DNA cut by each enzyme.