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Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436 Exercise 1
You want to map the positions of three genes ( X , Y , and Z ) all found on one chromosome in Drosophila. Each gene has one dominant allele and one recessive allele. You perform the three different two-factor crosses (Cross 1: XY and xy , Cross 2: YZ and yz, and Cross 3: XZ and xz ). Assume all crosses are between diploid flies homozygous for the alleles of these genes. You observe 7% recombinants in the first cross, 20% recombinants in the second cross, and 13% recombinants in the third cross. Draw a map placing the genes in the proper order and give the distance between each gene in map units (m.u.).
Explanation
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The recombination frequency (RF) is the probability of a cross-over between the two genes that are present on the same chromosome. The formula for the recombination frequency can be mentioned as follows:
1% of recombination frequency is equivalent to 1 map unit (m.u) or one centimorgan (cM).
From the given data, the recombination frequency between X and Y is 7%, Y and Z is 20% and X and Z is 13%. In general, a gene pair with the highest RF would likely be located farthest from each other. So, the gene map is as follows:
1% of recombination frequency is equivalent to 1 map unit (m.u) or one centimorgan (cM).From the given data, the recombination frequency between X and Y is 7%, Y and Z is 20% and X and Z is 13%. In general, a gene pair with the highest RF would likely be located farthest from each other. So, the gene map is as follows:
Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
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