Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 13

Using the same experimental setup as in the original Meselson and Stahl experiment (see Figs. 2-9 and 2-10), predict the bands (heavy, light, and/or intermediate) that you would observe after one round of replication if DNA polymerase replicated the bacterial genome by the dispersive model of replication.
B. Using the same experimental setup as in the original Meselson and Stahl experiment, predict the bands (heavy, light, and/or intermediate) that you would observe after one round of replication if DNA polymerase replicated the bacterial genome by the conservative model of replication.
C. How many rounds of replication would the original Meselson and Stahl experiment include to distinguish between the three models of replication (dispersive, conservative, semiconservative)? Explain your answer.