Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 5

Review the experiment and data depicted in Figure 2-19. In terms of the experimental setup, explain why the values for radioactivity per length of peptide are not equal for each full-length peptide isolated.
B. Predict how the data shown in the graph would change if the trypsin cleavage step did not take place.
C. Predict how the data shown in the graph would change if proteins were translated in the carboxy-terminal to amino-terminal direction. Explain why you would see that change.