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Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436 Exercise 8
The restriction endonuclease, XhoI, recognizes the sequence 5?-CTCGAG-3? and cleaves between the C and T on each strand.
A. What is the calculated frequency of this sequence occurring in a genome?
B. The restriction endonuclease, SalI, recognizes the sequence 5?-GTCGAC-3? and cleaves between the G and T on each strand. Do you think the sticky ends produced after XhoI and SalI cleavage could adhere to each other? Explain your choice.
A. What is the calculated frequency of this sequence occurring in a genome?
B. The restriction endonuclease, SalI, recognizes the sequence 5?-GTCGAC-3? and cleaves between the G and T on each strand. Do you think the sticky ends produced after XhoI and SalI cleavage could adhere to each other? Explain your choice.
Explanation
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A)Restriction endonucleases are enzymes ...
Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
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