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Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436 Exercise 4
Three assays for testing interactions between protein and DNA are the electromobility shift assay (EMSA), DNA footprinting, and chromatin immunoprecipitation (ChIP).
A. In a DNA footprinting assay, explain why only one strand of the DNA can be end-labeled for the experiment to work.
B. Following the immunoprecipitation step in a chromatin immunoprecipitation (ChIP), explain how to identify the DNA sequences that remain bound to the protein of interest.
A. In a DNA footprinting assay, explain why only one strand of the DNA can be end-labeled for the experiment to work.
B. Following the immunoprecipitation step in a chromatin immunoprecipitation (ChIP), explain how to identify the DNA sequences that remain bound to the protein of interest.
Explanation
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A)The protein binding site in a DNA can ...
Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
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