Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 1

Consider a bacterial promoter with ?35 and ?10 elements. What assay is best to show that RNA polymerase binds at regions centered on the ?35 and ?10 positions upstream of the start site of transcription? (Reviewing Chapter 7 may help.)