Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 10

List the key steps necessary to select a ribozyme with ligase activity through Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Assume that you will complete multiple rounds of selection and that you want to diversify the pool each round. You add an RNA molecule that includes a sequence tag to the mixture of your potential ribozymes. If a ribozyme ligates the tag to its own 5? end, the ligating ribozyme can be purified out from the mix.