Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 6

A list of the steps for chromatin immunoprecipitation (ChIP) in the incorrect order follows. Provide the proper order for the steps of ChIP by listing the letter of each step.
a. Immunoprecipitate DNA-protein complex.
b. Amplify DNA by PCR.
c. Add antibody specific to one protein.
d. Cross-link proteins to DNA fragments.
e. Remove proteins.
B. A researcher chose to perform a ChIP assay using an antibody to protein X and PCR primers specific to the region including gene Y. Give an example of the question asked by the researcher that led to these choices.