Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann

Exercise 9

To use RNAi as an experimental tool, initially researchers delivered the dsRNA to worms by feeding the worms E. coli engineered to express the dsRNA. Name two reasons why directly delivering the dsRNA does not work as efficiently in mammalian cells and describe how researchers overcome this problem.