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Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
Edition 7ISBN: 9780321762436 Exercise 12
You want to silence the expression of a target gene using an shRNA.
A. Name several possible assays to test expression of the target gene.
B. Describe appropriate controls for an assay testing protein levels of the target gene product and proving that down-regulation is due to the shRNA involved.
A. Name several possible assays to test expression of the target gene.
B. Describe appropriate controls for an assay testing protein levels of the target gene product and proving that down-regulation is due to the shRNA involved.
Explanation
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In order to silence the expression of th...
Molecular Biology of the Gene 7th Edition by Richard Losick, James Watson, Michael Levine, Tamara Baker, Alexander Gann
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