Modern Experimental Biochemistry 3rd Edition by Rodney Boyer

Exercise 2

Design a "shortcut" method for preparing the phosphate buffer in Study Problem. Hint: You need only NaH₂ PO 4 a solution of NaOH, and a pH meter.
You need to prepare a buffer for biochemistry lab. The required solution is 0.5 M sodium phosphate, pH 7.0. Use the Henderson-Hasselbalch equation to calculate the number of moles and grams of monobasic sodium phosphate (NaH₂ PO 4 ) and dibasic sodium phosphate (Na 2 HPO 4 ) necessary to make 1 liter of the solution.