Modern Experimental Biochemistry 3rd Edition by Rodney Boyer

Exercise 1

Amino acid analyzers are instruments that automatically separate amino acids by cation-exchange chromatography. Predict the order of elution (first to last) for each of the following sets of amino acids at pH = 4.
(a) Gly, Asp, His
(b) Arg, Glu, Ala
(c) Phe, His, Glu