Modern Experimental Biochemistry 3rd Edition by Rodney Boyer

Exercise 2

In a laboratory experiment, you are asked to determine the molar con­centration of a solution of an unknown compound, X. The solution di­luted in half by water (1 mL of X and 1 mL of H₂ O) has an absorbance at 425 nm of 0.8 and a molar extinction coefficient of 1.5 × 10 3 M ?1 cm ?1. What is the molar concentration of the original solution of X?