Fundamentals Of Microbiology 10th Edition by Jeffrey Pommerville

Exercise 13

Answer the following questions that pertain to (1) transcription and translation, and (2) mutations. Use the genetic code (Table).You are interested in identifying mutants of E. coli; specifically mutations occurring in the promoter and operator regions of the lac operon such that their respective molecules cannot bind to the region. You have agar plates containing lactose or glucose as the energy source. How can plating the potential mutants on these media help you identify
(a) promoter region mutants and
(b) operator region mutants
Table: The Genetic Code Decoder