Molecular Biology Of The Cell 6th Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Exercise 12

Which statements are true? explain why or why not.
-In a subset of voltage-gated channels, the N-terminus of each subunit acts like a tethered ball that occludes the cytoplasmic end of the pore soon after it opens, thereby inactivating the channel. This "ball-and- chain" model for the rapid inactivation of voltage-gated channels has been elegantly supported for the shaker channel from Drosophila melanogaster. (The shaker channel in Drosophila is named after a mutant form that causes excitable behavior-even anesthetized flies keep twitching.) Deletion of the N-terminal amino acids from the normal shaker channel gives rise to a channel that opens in response to membrane depolarization, but stays open instead of rapidly closing as the normal chan- nel does. A peptide (MAAVAGLYGLGEDRQHRKKQ) that corresponds to the deleted N-terminus can inactivate the open channel at Is the concentration of free peptide (100 µM) that is required to inactivate the defective channel anywhere near the local concentration of the tethered ball on a nor- mal channel? Assume that the tethered ball can explore a hemisphere with a radius of 21.4 nm, which is the length of the polypeptide "chain" (Figure Q11-2). Calculate the concentration for one ball in this hemisphere. How does that value compare with the con- centration of free peptide needed to inactivate the chan- nel?