Molecular Biology Of The Cell 6th Edition by Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Exercise 2

In contrast to their similar brain abnormalities, newborn mice deficient in Apaf1 or caspase-9 have dis- tinctive abnormalities in their paws. Apaf1-deficient mice fail to eliminate the webs between their developing digits, whereas caspase-9-deficient mice have normally formed digits (Figure Q18-1). If Apaf1 and caspase-9 function in the same apoptotic pathway, how is it possible for these deficient mice to differ in web-cell apoptosis?
B. Release of cytochrome GFP is detected as a change from a punctate to a diffuse pattern of fluorescence. Times after UV exposure are indicated as hours:minutes below the individual panels. Which model for cytochrome c release do these observa- tions support? Explain your reasoning.