Biomedical Engineering: Bridging Medicine and Technology 1st Edition by Veronique Tran,Mark Saltzman

Exercise 14

You want to fill two culture chambers (surface area = 10 cm2) with freshly dissected cells, the first at a plating density of 50,000 cells/cm2 and the second at 750,000 cells/cm2. A representative sample from the primary cell suspension was first diluted 10:1 (total volume/sample volume) and then counted on a hemocytometer (see Figure 5.24). What volume of the original cell suspension must be added to each culture to achieve the desired plating density?