Biomedical Engineering: Bridging Medicine and Technology 1st Edition by Veronique Tran,Mark Saltzman

Exercise 4

You are culturing insulinoma cells (called INS-1) for in vitro experiments.
These cells have a doubling time of 36 hours.
a. What is the specific growth rate of the cells?
b. Plot a graph describing the growth behavior of these cells over time.
c. A T-flask is seeded with 10 × 106 cells; how many days can you wait
until you have to passage the cells? [Note: Assume that a confluent T-flask
contains approximately 75 × 106 cells.]