Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Question

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Exercise 1

An enzyme ATPase has a molecular weight of $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ daltons, $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ value of 10-4 M, and a K₂
value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the
following: $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ which can also be represented as $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics
are first order: $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ where $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ is the initial enzyme concentration and $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ In an experiment with a par-
tially pure enzyme preparation, $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ of total crude protein (containing enzyme) is added to a
1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac-
tion ends $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ and the inorganic phosphate $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ concentration is found to be 0.002 M,
which was initially zero. What fraction of the crude protein preparation was the enzyme?
Hint: Since $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$ the reaction rate can be represented by $An enzyme ATPase has a molecular weight of daltons, value of 10-4 M, and a K₂ value of K₂ = 104 molecules ATP/min molecule enzyme at 37∞C. The reaction catalyzed is the following: which can also be represented as where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order: where is the initial enzyme concentration and In an experiment with a par- tially pure enzyme preparation, of total crude protein (containing enzyme) is added to a 1 ml reaction mixture containing 0.02 M ATP and incubated at 37∞C. After 12 hours the reac- tion ends and the inorganic phosphate concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since the reaction rate can be represented by$

Explanation

Verified

The catalyzed reaction is as follows: T...