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Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086 Exercise 9
The enzyme, urease, is immobilized in Ca-alginate beads 2 mm in diameter. When the urea
concentration in the bulk liquid is 0.5 mM the rate of urea hydrolysis is v = 10 mmoles-l-h.
Diffusivity of urea in Ca-alginate beads is
and the Michaelis constant
for the enzyme is
By neglecting the liquid film resistance on the beads (i.e., 
determine the following:
a. Maximum rate of hydrolysis
Thiele modulus
and effectiveness factor
b. What would be the
values for a particle size of Dp = 4 mm?
Hint: Assume
for large values of
concentration in the bulk liquid is 0.5 mM the rate of urea hydrolysis is v = 10 mmoles-l-h.
Diffusivity of urea in Ca-alginate beads is
and the Michaelis constantfor the enzyme is
By neglecting the liquid film resistance on the beads (i.e., determine the following: a. Maximum rate of hydrolysis
Thiele modulus and effectiveness factor b. What would be the
values for a particle size of Dp = 4 mm?Hint: Assume
for large values ofExplanation
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The enzymes are the biological catalysts...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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