Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 14

Two enzymes are both immobilized on the same flat, nonporous surface. For enzyme A the sub-
strate is S₁. For enzyme B the substrate is S₂. The product of the first reaction is S₂. That is:
a. Figure 3.P1 depicts the rate of the first reaction on the surface as a function of local con-
centrations of S₁. If the bulk concentration of S₁ is 100 mg/l and the mass transfer coeffi-
cient is
cm/s, what is the rate of consumption of S₁ for a
surface? What is
the surface concentration of S₁?
b. The rate of the second reaction is:


where
The bulk concentra-
tion of
is maintained as 5 mg/l and the mass transfer coefficient is the same for

and S₂. Calculate
and the rate of formation of P (assuming all stoichiometric coeffi-
cients are one).