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Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086 Exercise 8
You are asked by your boss to produce a human protein in E. coli. Because you have learned
some of the differences in the way that procaryotes and eucaryotes make proteins, you worry
about at least two factors that could complicate production of an authentic protein for human
use.
a) What complication might you worry about if the human DNA encoding the protein were
placed directly in E. coli?
b) Assume that the correct primary sequence of amino acids has been produced. What post-
translational steps do you worry about and why?
some of the differences in the way that procaryotes and eucaryotes make proteins, you worry
about at least two factors that could complicate production of an authentic protein for human
use.
a) What complication might you worry about if the human DNA encoding the protein were
placed directly in E. coli?
b) Assume that the correct primary sequence of amino acids has been produced. What post-
translational steps do you worry about and why?
Explanation
Verified
Verified
a) When a human protein has produced in ...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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