Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 2

The growth of baker's yeast (S. cerevisiae) on glucose may be simply described by the fol-
lowing equation: In a batch reactor of volume l, the final desired yeast concentration is 50 gdw/l.
Using the above reaction stoichiometry:
a. Determine the concentration and total amount of glucose and (NH₄)2SO₄ in the nutrient
medium.
b. Determine the yield coefficients
(biomass/glucose) and
(biomass/oxygen).
c. Determine the total amount of oxygen required.
d. If the rate of growth at exponential phase is
gdw/l-h, determine the rate of oxygen
consumption

e. Calculate the heat-removal requirements for the reactor (recall eq. 6.26).