Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 8

In a two-stage chemostat system, the volumes of the first and second reactors are and = 300 l, respectively. The first reactor is used for biomass production and the second is
for a secondary metabolite formation. The feed flow rate to the first reactor is F = 100 l/h,
and the glucose concentration in the feed is S = 5.0 g/l. Use the following constants for the
cells.
a. Determine cell and glucose concentrations in the effluent of the first stage.
b. Assume that growth is negligible in the second stage and the specific rate of product for-
mation is
Determine the product and sub-
strate concentrations in the effluent of the second reactor.