Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 10

Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition
of glucose solution to the culture medium. The initial culture volume at quasi-steady state is and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h.
Glucose concentration in the feed solution and initial cell concentration are = 300 g/l and espectively. The kinetic and yield coefficients of the organism are glucose.
a. Determine the culture volume at t = 10 h.
b. Determine the concentration of glucose at t = 10 h at quasi-steady state.
c. Determine the concentration and total amount of cells at quasi-steady state when t = 10 h.
d. If
product/g cells h and
determine the product concentration in
the vessel at t = 10 h.