
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086 Exercise 1
The temperature history of the heating and cooling of a 40,000-l tank during sterilization of
medium is: 0 to 15 min, T = 85°C; 15 to 40 min, T = 121°C; 40 to 50 min, T = 85°C; 50 to 60
min,
min, T = 30°C. The medium contains vitamins, the most fragile of the vi-
tamins has an activation energy for destruction of 10 kcal/g-mol, and the value of
(see eq.
10.18) is 1 ·
Assume vitamin destruction is first order and the initial concentration
is 50 mg/l. R is 1.99 cal/g-mol-°K. The medium contains 2.5 ◊ 103 spores/l. The spores have
an
kcal/g-mol, and
Estimate: (a) the probability of a suc-
cessful sterilization, and (b) what fraction of the vitamin remains active?
medium is: 0 to 15 min, T = 85°C; 15 to 40 min, T = 121°C; 40 to 50 min, T = 85°C; 50 to 60
min,

tamins has an activation energy for destruction of 10 kcal/g-mol, and the value of

10.18) is 1 ·

is 50 mg/l. R is 1.99 cal/g-mol-°K. The medium contains 2.5 ◊ 103 spores/l. The spores have
an


cessful sterilization, and (b) what fraction of the vitamin remains active?
Explanation
a) With resepect to sterilization the he...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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