Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 10

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for
production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately
X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are respectively. The rate constant for Mab formation is Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.
a. Determine the volume and the height of the packed bed for 95% glucose conversion. Bed
diameter is
Neglect the growth of the hybridomas and assume first order ki-
netics.
b. If
Mab/g glu, determine the effluent Mab concentration and the productivity
of the system.