Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 4

C. roseus cells immobilized in Ca-alginate beads of diameter 0.5 mm are used for production
of indole alkaloids (IA) in a fluidized-bed bioreactor. The rate limiting nutrient is glucose
and no intraparticle diffusion limitations exist. Use the following data: Flow rate of the feed:
Q = 1 l/h, Glucose in the feed: Plant Cell Concentration: X = 6 g/l reac. The rate
constant for IA formation: Column diameter: Growth is negligible and Monod kinetics is valid. Determine the following:
a. For 95% glucose conversion determine required hydraulic residence time, volume, and
the height of the column
b. If
glu, determine IA concentration in the effluent and the productivity.