Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Question

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Edition 2ISBN: 9780130819086

Exercise 2

Consider an industrial-scale batch fermentation. A 10,000 l fermenter with $Consider an industrial-scale batch fermentation. A 10,000 l fermenter with cells/ml is the desired scale-up operation. Inoculum for the large tank is brought through a series of seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume that a colony plasmid-containing cells) is picked and placed in a test tube with 1 ml of medium. Calculate how many generations will be required to achieve the required cell den- sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells$ cells/ml
is the desired scale-up operation. Inoculum for the large tank is brought through a series of
seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume
that a colony $Consider an industrial-scale batch fermentation. A 10,000 l fermenter with cells/ml is the desired scale-up operation. Inoculum for the large tank is brought through a series of seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume that a colony plasmid-containing cells) is picked and placed in a test tube with 1 ml of medium. Calculate how many generations will be required to achieve the required cell den- sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells$ plasmid-containing cells) is picked and placed in a test tube with 1 ml of
medium. Calculate how many generations will be required to achieve the required cell den-
sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells $Consider an industrial-scale batch fermentation. A 10,000 l fermenter with cells/ml is the desired scale-up operation. Inoculum for the large tank is brought through a series of seed tanks and flasks, beginning with a single pure colony growing on an agar slant. Assume that a colony plasmid-containing cells) is picked and placed in a test tube with 1 ml of medium. Calculate how many generations will be required to achieve the required cell den- sity in the 10,000 l fermenter. What fraction of the total population will be plasmid-free cells$

Explanation

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According to the given data:
In industri...