Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler

Exercise 3

Assume that you have been assigned to a team to produce human epidermal growth factor
(hEGF). A small peptide, hEGF speeds wound healing and may be useful in treating ulcers. A
market size of 50 to 500 kg/yr has been estimated. Posttranslational processing is not essen-
tial to the value of the product. It is a secreted product in the natural host cell. Discuss what
recommendations you would make to the molecular-biology team leader for the choice of
host cell and the design of a reactor. Make your recommendations from the perspective of
what is desirable to make an effective process. You should point out any potential prob-
lems with the solution you have proposed, as well as defend why your approach should be
advantageous.