Suppose Drug a and Drug B Are to Be Separated

Multiple Choice

Suppose drug A and drug B are to be separated by anion-exchange chromatography.Drug A is an acid with a pKₐ of 8.0, and drug B is an acid with a pKₐ of 4.0.Following the proper steps to place the mixture of drugs onto the anion-exchange phase, the pH of the mobile phase should be adjusted to ______ to elute drug A only.Then the pH of the mobile phase should be adjusted to _____ to elute drug B from the stationary phase.

A) 4.0, 8.0
B) 8.0, 4.0
C) 10.0, 6.0
D) 6.0, 2.0