Deck 4: Use of the Microscope

Question 1

The ability to distinguish between two objects located close to each other is called ________.

A) resolution
B) depth of field
C) total magnification
D) field of view

Answer 1

A

Question 2

The ________ is the eyepiece where you place your eyes to observe the specimen.

A) condenser
B) nosepiece
C) objective lenses
D) ocular lens

Answer 2

D

Question 3

Which of the following is NOT a step in preparing a wet-mount?

A) Place the specimen on the slide and add a small drop of water to it.
B) Use the coarse adjustment knob to move the objective lens as close to the specimen as possible without touching the slide.
C) Do not use a coverslip with a wet-mount preparation.
D) Using tweezers or your fingers, touch the water stain with the edge of the coverslip.

Answer 3

C

Question 4

All of the following are ways to best control the magnification of the microscope EXCEPT which statement?

A) To examine part of the specimen at higher magnification, move that part of the slide to the center over the aperture before changing to a higher-magnification lens.
B) To examine part of the specimen at higher magnification, move that part of the specimen off to the side.
C) Always use low magnification during your initial observation.
D) Use a drop of immersion oil to look at the specimen under the highest magnification.

Question 5

The oil immersion objective is typically ________.

A) 45X
B) 1000X
C) 10,000X
D) 100X

Question 6

The following are all needed to make and view a wet-mount EXCEPT ________.

A) a coverslip
B) water
C) a slide
D) glue, for mounting the coverslip

Question 7

What part of the microscope is mounted on the nosepiece?

A) objective lenses
B) focus knobs
C) iris diaphragm
D) condenser

Question 8

As magnification increases ________.

A) the field diameter increases
B) the field diameter decreases

Question 9

Which of the following is NOT one of the four basic steps in successfully viewing a specimen under the microscope?

A) setup
B) placing the specimen on the stage of the microscope
C) focusing
D) magnification

Question 10

As the magnification ________, the field diameter ________.

A) increases; increases
B) decreases; decreases
C) increases; decreases
D) none of the above

Question 11

Depth of field is ________.

A) greatest at high power and decreases as magnification increases
B) greatest at low power and increases as magnification increases
C) greatest at low power and decreases as magnification increases
D) greatest at high power and increases as magnification increases

Question 12

All of the following are correct steps in focusing on a specimen under the microscope EXCEPT which statement?

A) Once the image is clear, use the fine adjustment knob to focus on small details.
B) When you are ready to change magnification, do not move the adjustment knobs before changing the objective lens.
C) If two images are visible, slowly move the body tubes closer together until you see with both eyes open, a single circular field of view.
D) Start with a high power objective lens when focusing.

Question 13

The following are all guidelines for proper use of a microscope EXCEPT which statement?

A) Completely unwind the electrical plug before plugging it in.
B) Store the microscope with the low-power objective lens in position.
C) When cleaning the lens of a microscope, use water and a paper towel.
D) Carry the microscope with two hands at all times.

Question 14

The focal plane thickness ________ as magnification ________.

A) decrease; decreases
B) increases; increases
C) decreases; increases
D) none of the above

Question 15

The ________ is the supportive frame of the microscope that joins the body tube to the base.

A) body tube
B) base
C) arm
D) nosepiece

Question 16

All of the following are steps in setting up the microscope in order to view a specimen EXCEPT ________.

A) plugging in the electrical cord and turning the microscope lamp on
B) focusing
C) checking the position of the condenser
D) positioning the low-magnification objective lens over the aperture

Question 17

The following are all procedures to measure the field diameter EXCEPT which statement?

A) Position the slide so that a vertical line on the paper lines up with the edge of the field.
B) Count the number of millimeters across the field to measure the field diameter.
C) Use your low-power measured field diameter to calculate the field diameter for high power.
D) Position the slide so that the horizontal line on the paper is on the top of the field.

Question 18

The in-focus thickness of a specimen viewed under a microscope is called the ________.

A) field of view
B) resolution
C) focal plane
D) depth of field

Question 19

If the field diameter is 8mm and an object occupies one-half of the field, the object is approximately ________ wide.

A) 6mm
B) 2mm
C) 7mm
D) 4mm

Question 20

The ________ is a small lens under the stage that narrows the beam of light and directs it through the specimen on the slide.

A) ocular lens
B) iris diaphragm
C) condenser
D) objective lens

Question 21

All of the following are guidelines for using the oil-immersion lens EXCEPT which statement?

A) Once your slide is in position place a drop of oil on the slide and focus first at low power.
B) Place the slide on the microscope and focus first on low power, then medium and the high dry magnifications.
C) Once the oil is applied and the oil-immersion objective lens is moved in place, focus the lens with the fine focus knob.
D) Once the oil-immersion lens is in place, swing the objective away from the slide and add a small drop of oil

Question 22

All of the following are guidelines for cleaning the oil-immersion lens EXCEPT which statement?

A) Use a paper towel to wipe the oil off the lens.
B) Use the coarse focus knob and move the objective lens away from the slide to create a working space for cleaning.
C) Use a lens tissue to wipe the oil off the lens.
D) Place a drop of lens-cleaning solution on the paper and completely remove any remaining oil.