Deck 3: Cell Structure and Function in Bacteria and Archaea

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Question
What is the biological function of endospores?

A) They are bacterial reproductive structures.
B) They are formed as evaginations of the bacterial cell walls.
C) They enable organisms to endure extremes of temperature, drying, and nutrient depletion.
D) All of the above are endospore functions.
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Question
Nutrients and waste products pass into and out of a cell in proportion to the cell's size.

A) direct
B) logarithmic
C) inverse
D) exponential
Question
The cytoplasmic membrane could best be described as

A) a passive conduit for intracellular transport.
B) an impermeable barrier.
C) a highly selective permeability barrier.
D) slightly permeable to water but impermeable to metabolic solutes.
Question
One of the many types of proteins found in the cytoplasmic membrane is involved in the chemotactic response and is called a

A) porin.
B) binding protein.
C) hydrolytic enzyme.
D) chemoreceptor.
Question
Translocases are specific for the transport of

A) nucleic acids.
B) carbohydrates.
C) proteins.
D) lipids.
Question
In gram- positive Bacteria, the cell wall may be up to peptidoglycan.

A) 72%
B) 25%
C) 90%
D) 48%
Question
Carrier- mediated transport is necessary when

A) the level of nutrients in nature is very low.
B) diffusion will not allow adequate amounts of a substance to enter the cell.
C) movement into the cell is against a concentration gradient.
D) All of the above are true.
Question
The terms "run" and "tumble" are generally associated with

A) cell membrane structures.
B) clustering properties of certain rod- shaped bacteria.
C) movement of the cell.
D) cell wall fluidity.
Question
The tetraether molecule within the membrane structure of the yields a lipid .

A) Archaea / bilayer
B) Bacteria / monolayer
C) Archaea / monolayer
D) Bacteria / bilayer
Question
Which is a function of the cytoplasmic membrane in prokaryotes?

A) It is an anchor for many proteins involved in bioenergetic reactions and transport.
B) It functions as a permeability barrier.
C) It is a major site of energy conservation.
D) All of these are functions of the cytoplasmic membrane.
Question
The polysaccharide pseudomurein is present in the walls of

A) some Archaea.
B) Bacteria and Archaea.
C) Eukarya.
D) Bacteria.
Question
Negatively charged molecules that are partially responsible for the negative charge of the gram- positive bacterial cell surface are

A) teichoic acids.
B) peptide interbridges.
C) phospholipids.
D) diaminopimelic acids.
Question
Which of these statements is TRUE?

A) The gram- positive wall contains a thick peptidoglycan layer and is more complex than the gram- negative wall.
B) The gram- positive wall contains a thick peptidoglycan layer but is about as complex as the gram- negative wall.
C) The gram- negative wall contains a thick peptidoglycan layer and is more complex than the gram- positive wall.
D) The gram- positive wall contains a thick peptidoglycan layer but is less complex than the gram- negative wall.
Question
An endotoxin is

A) a toxin known for its primary attack on the epidermis of mammals.
B) the toxin produced in the periplasm of most bacteria.
C) the toxic portion of the LPS.
D) a toxin produced within archaeal cells.
Question
Hydrolytic enzymes function in the

A) chemotactic response, particularly in gram- negative Bacteria.
B) regeneration of the periplasm.
C) transport of substrates within the cell.
D) initial degradation of nutrients.
Question
The prokaryotic transport system that involves a substrate- binding protein, a membrane- integrated transporter, and an ATP- hydrolyzing protein is

A) group translocation.
B) the ABC system.
C) symport.
D) simple transport.
Question
Regarding membrane linkages, which statement is TRUE?

A) Archaea have ester linkages; Eukarya have ether linkages.
B) Bacteria and Eukarya have ester linkages; Archaea linkages are not yet known.
C) Bacteria and Eukarya have ester linkages; Archaea have ether linkages.
D) Eukarya have ester linkages; Bacteria have ether linkages.
Question
When does endospore formation commence?

A) following bacterial death
B) when bacterial growth ceases due to limitation of an essential nutrient
C) when bacteria are dividing exponentially
D) when the bacterium is undergoing binary fission
Question
Bacteria with appendages

A) have filaments that are commonly called appendages.
B) have the basic characteristics of eukaryotic cells.
C) possess tubular or stalk- like extensions of their cells.
D) do not exist.
Question
Which statement is TRUE?

A) The phosphotransferase system has nothing to do with group translocation.
B) The phosphotransferase system is synonymous with group translocation.
C) The phosphotransferase system is a type of group translocation.
D) Group translocation is a part of the phosphotransferase system.
Question
Which of the following statements is/are TRUE?

A) Fimbriae enable cells to stick to surfaces.
B) Pili serve as receptors and facilitate genetic exchange between prokaryotic cells.
C) Fimbriae are generally shorter and more numerous than flagella.
D) All of the above are true.
Question
Aquaporins are

A) molecules that prevent water from crossing a membrane.
B) cations bound to water molecules.
C) water transport proteins.
D) enzymes involved in the generation of water within cells.
Question
Sterols are virtually always

A) absent in eukaryotic membranes but present in prokaryotic membranes.
B) present in eukaryotic membranes but absent in prokaryotic membranes.
C) absent in both eukaryotic and prokaryotic membranes.
D) present in both eukaryotic and prokaryotic membranes.
Question
The term given to the phospholipid bilayer and the proteins embedded within it is
.
Question
Glycogen granules

A) cannot be detected by light microscopy, even if iodine is added.
B) function as storage depots for carbon and energy.
C) are derived directly from either PHB or PHA.
D) None of the above applies to glycogen granules.
Question
Although the inner leaflet of the gram- negative outer membrane contains phospholipids, the outer leaflet of the outer membrane contains

A) lipopolysaccharide (LPS).
B) pseudopeptidoglycan.
C) poly- fi- hydroxybutyric acid (PHB).
D) lipoteichoic acids.
Question
Which statement is TRUE?

A) Lophotrichous flagella are tufts on the "ends" of bacterial cells; peritrichous flagella are individual flagella on the "ends" of bacterial cells.
B) Polar flagella are individual flagella on the "ends" of bacterial cells; peritrichous flagella are tufts on the "ends" of bacterial cells.
C) Peritrichous flagella are all over the bacterial cells; lophotrichous flagella are tufts on the "ends" of bacterial cells.
D) Polar flagella are all over the bacterial cells; lophotrichous flagella are individual flagella on the "ends" of bacterial cells.
Question
The proton motive force is responsible for driving many energy- requiring functions in the cell, including some forms of , , and biosynthesis of _ .
Question
The membrane of a gas vesicle is composed of

A) protein.
B) various phospholipids.
C) carbohydrate.
D) both glycoproteins and phospholipids.
Question
Twitching motility is an unusual form of motility in some bacteria that is a result of a class of surface structures known as .
Question
An organism of the genus Staphylococcus is , while an organism of the genus Spirochaeta is .

A) spherical / coiled
B) spherical / rod shaped
C) coiled / spherical
D) rod shaped / coiled
Question
Morphology is a term that refers to the of an organism.

A) appendages
B) attachment sites
C) size
D) shape
Question
Which of the following statements is FALSE?

A) Flagella rotate at a constant speed.
B) A flagellar protein subunit is flagellin.
C) In flagellar motion, the basal body acts as a motor.
D) The hook is the wider region at the base of the flagellum.
Question
A major function of prokaryotic gas vesicles is

A) to keep the cell's organelles separated during flagellar motion.
B) to serve as a reservoir for oxygen and carbon dioxide.
C) to confer buoyancy on cells by decreasing their density.
D) none of the above.
Question
Bacteria stain as gram- positive or gram- negative because of differences in the cell

A) wall.
B) nucleus.
C) chromosome.
D) cytoplasm.
Question
The lipopolysaccharide (LPS) layer is found ONLY in the cell wall of

A) gram- negative Bacteria.
B) Eukarya.
C) gram- positive Bacteria.
D) Archaea.
Question
A general term to describe intracellular accumulations of polyphosphate, elemental sulfur, or magnetosomes is a(n) .
Question
Some of the intestinal symptoms elicited by pathogens such as Salmonella, Shigella, and
Escherichia are due to the presence of .
Question
The periplasm is a(n)

A) part of the outer cell membrane of gram- negative organisms.
B) alternate name for the inner cell membrane of any prokaryotic cell.
C) region between the cytoplasmic membrane and the outer membrane of gram- negative Bacteria.
D) part of the inner cell membrane of gram- negative organisms.
Question
The energy source derived from the charge separation across the cytoplasmic membrane is referred to as the .
Question
Small acid- soluble proteins (SASPs) are found in high numbers within the core of
, which help to protect DNA from . They also serve as a(n) source during outgrowth.
Question
A phototrophic bacterium swims outside the illuminated field of view of the microscope, reverses direction, and scurries back into the lighted area. This is an example of .
Question
The lipid portion of the lipopolysaccharide (LPS) is known as lipid A.
Question
An individual rod- shaped bacterium is also called a(n) morphology, while two or more of these are collectively described as _ _. Similarly, one is a spherical bacterium, and two or more are termed .
Question
As a general statement, inclusions function as energy reserves or as a reservoir of structural building blocks.
Question
A purple bacterial cell is interpreted as gram- positive when it forms insoluble crystal violet- iodine complexes within the cell during the Gram stain.
Question
Lysozyme is an enzyme that can ultimately lyse and kill bacterial cells by breaking
fi- 1,4- glycosidic bonds in peptidoglycan.
Question
In general, swimming is performed with flagella, whereas gliding uses other cellular components such as pili.
Question
Porins are channels in the outer membranes of gram- positive Bacteria.
Question
In general, lipids in the archaeal membrane lack true fatty acids.
Question
The proton motive force helps drive cellular functions that require energy by maintaining a positively charged side of the cell and the other negatively charged.
Question
Eukaryotes have in their cytoplasmic membranes, which serve to strengthen and stabilize the membrane and make it less flexible. Many Bacteria have similar molecules, known as _ , in their membranes that likely have a similar role.
Question
Some membrane proteins are involved in bioenergetic reactions, while others are involved in membrane transport.
Question
Smaller prokaryotic cells generally grow faster than larger ones due to a higher surface- area- to- volume ratio.
Question
Hopanoids strengthen the plasma membrane in prokaryotes and form a highly rigid membrane, analogous to the function of cholesterol in animals.
Question
The rigid layer that is present in the cell walls of Bacteria that is primarily responsible for the strength of the wall is known as .
Question
Pathogenic bacteria that contain S- layers are protected against host defense mechanisms.
Question
Despite the invariance of the peptidoglycan backbone's structure, there are more than 100 different types of peptidoglycan.
Question
Teichoic acids are commonly found in gram- negative cell walls.
Question
The peptide interbridge crosslinking is found ONLY in the cell walls of Bacteria.
Question
In phototaxis, photosynthetic organisms move away from a light source.
Question
Elaborate on why the discovery of endospores was important to microbiology.
Question
Describe the processes of endospore formation and germination.
Question
What is the evolutionary advantage of the monolayer often found in archaeal membranes?
Question
Chemotaxis is a sensory response affecting flagellar function.
Question
Describe the growth of a flagellum.
Question
Explain what would happen to a motile bacterium undergoing chemotaxis if the Fli proteins suddenly ceased to function.
Question
Organisms may respond to temporal rather than spatial gradients.
Question
Explain the differences between uniporters, symporters, and antiporters.
Question
Compare and contrast fimbriae and pili.
Question
Compare and contrast a capsule and a slime layer in terms of composition, organization, and function.
Question
Photoreceptors are analogous to chemoreceptors in that they are both proteinaceous sensors.
Question
Explain why prokaryotes tend to adapt more rapidly to changing environmental conditions and more easily exploit new habitats than do eukaryotes.
Question
Compare and contrast the location and activities of periplasm- binding proteins of ABC transport systems in gram- negative and gram- positive Bacteria.
Question
Compare and contrast aerotaxis and osmotaxis.
Question
Both hydrophilic and charged molecules readily diffuse through the cytoplasmic membrane.
Question
Speculate on why all prokaryotic transport systems require energy in some form or another.
Question
The existence of nanobacteria was confirmed in 2006.
Question
Poly- fi- hydroxybutyric acid (PHB) is a carbon- and energy- storing polymer.
Question
ABC transport systems are present in both gram- positive and gram- negative Bacteria.
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Deck 3: Cell Structure and Function in Bacteria and Archaea
1
What is the biological function of endospores?

A) They are bacterial reproductive structures.
B) They are formed as evaginations of the bacterial cell walls.
C) They enable organisms to endure extremes of temperature, drying, and nutrient depletion.
D) All of the above are endospore functions.
C
2
Nutrients and waste products pass into and out of a cell in proportion to the cell's size.

A) direct
B) logarithmic
C) inverse
D) exponential
C
3
The cytoplasmic membrane could best be described as

A) a passive conduit for intracellular transport.
B) an impermeable barrier.
C) a highly selective permeability barrier.
D) slightly permeable to water but impermeable to metabolic solutes.
C
4
One of the many types of proteins found in the cytoplasmic membrane is involved in the chemotactic response and is called a

A) porin.
B) binding protein.
C) hydrolytic enzyme.
D) chemoreceptor.
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k this deck
5
Translocases are specific for the transport of

A) nucleic acids.
B) carbohydrates.
C) proteins.
D) lipids.
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k this deck
6
In gram- positive Bacteria, the cell wall may be up to peptidoglycan.

A) 72%
B) 25%
C) 90%
D) 48%
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7
Carrier- mediated transport is necessary when

A) the level of nutrients in nature is very low.
B) diffusion will not allow adequate amounts of a substance to enter the cell.
C) movement into the cell is against a concentration gradient.
D) All of the above are true.
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8
The terms "run" and "tumble" are generally associated with

A) cell membrane structures.
B) clustering properties of certain rod- shaped bacteria.
C) movement of the cell.
D) cell wall fluidity.
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k this deck
9
The tetraether molecule within the membrane structure of the yields a lipid .

A) Archaea / bilayer
B) Bacteria / monolayer
C) Archaea / monolayer
D) Bacteria / bilayer
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k this deck
10
Which is a function of the cytoplasmic membrane in prokaryotes?

A) It is an anchor for many proteins involved in bioenergetic reactions and transport.
B) It functions as a permeability barrier.
C) It is a major site of energy conservation.
D) All of these are functions of the cytoplasmic membrane.
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k this deck
11
The polysaccharide pseudomurein is present in the walls of

A) some Archaea.
B) Bacteria and Archaea.
C) Eukarya.
D) Bacteria.
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12
Negatively charged molecules that are partially responsible for the negative charge of the gram- positive bacterial cell surface are

A) teichoic acids.
B) peptide interbridges.
C) phospholipids.
D) diaminopimelic acids.
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k this deck
13
Which of these statements is TRUE?

A) The gram- positive wall contains a thick peptidoglycan layer and is more complex than the gram- negative wall.
B) The gram- positive wall contains a thick peptidoglycan layer but is about as complex as the gram- negative wall.
C) The gram- negative wall contains a thick peptidoglycan layer and is more complex than the gram- positive wall.
D) The gram- positive wall contains a thick peptidoglycan layer but is less complex than the gram- negative wall.
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14
An endotoxin is

A) a toxin known for its primary attack on the epidermis of mammals.
B) the toxin produced in the periplasm of most bacteria.
C) the toxic portion of the LPS.
D) a toxin produced within archaeal cells.
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k this deck
15
Hydrolytic enzymes function in the

A) chemotactic response, particularly in gram- negative Bacteria.
B) regeneration of the periplasm.
C) transport of substrates within the cell.
D) initial degradation of nutrients.
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k this deck
16
The prokaryotic transport system that involves a substrate- binding protein, a membrane- integrated transporter, and an ATP- hydrolyzing protein is

A) group translocation.
B) the ABC system.
C) symport.
D) simple transport.
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k this deck
17
Regarding membrane linkages, which statement is TRUE?

A) Archaea have ester linkages; Eukarya have ether linkages.
B) Bacteria and Eukarya have ester linkages; Archaea linkages are not yet known.
C) Bacteria and Eukarya have ester linkages; Archaea have ether linkages.
D) Eukarya have ester linkages; Bacteria have ether linkages.
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Unlock Deck
k this deck
18
When does endospore formation commence?

A) following bacterial death
B) when bacterial growth ceases due to limitation of an essential nutrient
C) when bacteria are dividing exponentially
D) when the bacterium is undergoing binary fission
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k this deck
19
Bacteria with appendages

A) have filaments that are commonly called appendages.
B) have the basic characteristics of eukaryotic cells.
C) possess tubular or stalk- like extensions of their cells.
D) do not exist.
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k this deck
20
Which statement is TRUE?

A) The phosphotransferase system has nothing to do with group translocation.
B) The phosphotransferase system is synonymous with group translocation.
C) The phosphotransferase system is a type of group translocation.
D) Group translocation is a part of the phosphotransferase system.
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Unlock Deck
k this deck
21
Which of the following statements is/are TRUE?

A) Fimbriae enable cells to stick to surfaces.
B) Pili serve as receptors and facilitate genetic exchange between prokaryotic cells.
C) Fimbriae are generally shorter and more numerous than flagella.
D) All of the above are true.
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Unlock Deck
k this deck
22
Aquaporins are

A) molecules that prevent water from crossing a membrane.
B) cations bound to water molecules.
C) water transport proteins.
D) enzymes involved in the generation of water within cells.
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k this deck
23
Sterols are virtually always

A) absent in eukaryotic membranes but present in prokaryotic membranes.
B) present in eukaryotic membranes but absent in prokaryotic membranes.
C) absent in both eukaryotic and prokaryotic membranes.
D) present in both eukaryotic and prokaryotic membranes.
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24
The term given to the phospholipid bilayer and the proteins embedded within it is
.
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k this deck
25
Glycogen granules

A) cannot be detected by light microscopy, even if iodine is added.
B) function as storage depots for carbon and energy.
C) are derived directly from either PHB or PHA.
D) None of the above applies to glycogen granules.
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Unlock Deck
k this deck
26
Although the inner leaflet of the gram- negative outer membrane contains phospholipids, the outer leaflet of the outer membrane contains

A) lipopolysaccharide (LPS).
B) pseudopeptidoglycan.
C) poly- fi- hydroxybutyric acid (PHB).
D) lipoteichoic acids.
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k this deck
27
Which statement is TRUE?

A) Lophotrichous flagella are tufts on the "ends" of bacterial cells; peritrichous flagella are individual flagella on the "ends" of bacterial cells.
B) Polar flagella are individual flagella on the "ends" of bacterial cells; peritrichous flagella are tufts on the "ends" of bacterial cells.
C) Peritrichous flagella are all over the bacterial cells; lophotrichous flagella are tufts on the "ends" of bacterial cells.
D) Polar flagella are all over the bacterial cells; lophotrichous flagella are individual flagella on the "ends" of bacterial cells.
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28
The proton motive force is responsible for driving many energy- requiring functions in the cell, including some forms of , , and biosynthesis of _ .
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Unlock Deck
k this deck
29
The membrane of a gas vesicle is composed of

A) protein.
B) various phospholipids.
C) carbohydrate.
D) both glycoproteins and phospholipids.
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k this deck
30
Twitching motility is an unusual form of motility in some bacteria that is a result of a class of surface structures known as .
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k this deck
31
An organism of the genus Staphylococcus is , while an organism of the genus Spirochaeta is .

A) spherical / coiled
B) spherical / rod shaped
C) coiled / spherical
D) rod shaped / coiled
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32
Morphology is a term that refers to the of an organism.

A) appendages
B) attachment sites
C) size
D) shape
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k this deck
33
Which of the following statements is FALSE?

A) Flagella rotate at a constant speed.
B) A flagellar protein subunit is flagellin.
C) In flagellar motion, the basal body acts as a motor.
D) The hook is the wider region at the base of the flagellum.
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34
A major function of prokaryotic gas vesicles is

A) to keep the cell's organelles separated during flagellar motion.
B) to serve as a reservoir for oxygen and carbon dioxide.
C) to confer buoyancy on cells by decreasing their density.
D) none of the above.
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k this deck
35
Bacteria stain as gram- positive or gram- negative because of differences in the cell

A) wall.
B) nucleus.
C) chromosome.
D) cytoplasm.
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Unlock Deck
k this deck
36
The lipopolysaccharide (LPS) layer is found ONLY in the cell wall of

A) gram- negative Bacteria.
B) Eukarya.
C) gram- positive Bacteria.
D) Archaea.
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k this deck
37
A general term to describe intracellular accumulations of polyphosphate, elemental sulfur, or magnetosomes is a(n) .
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k this deck
38
Some of the intestinal symptoms elicited by pathogens such as Salmonella, Shigella, and
Escherichia are due to the presence of .
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k this deck
39
The periplasm is a(n)

A) part of the outer cell membrane of gram- negative organisms.
B) alternate name for the inner cell membrane of any prokaryotic cell.
C) region between the cytoplasmic membrane and the outer membrane of gram- negative Bacteria.
D) part of the inner cell membrane of gram- negative organisms.
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k this deck
40
The energy source derived from the charge separation across the cytoplasmic membrane is referred to as the .
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k this deck
41
Small acid- soluble proteins (SASPs) are found in high numbers within the core of
, which help to protect DNA from . They also serve as a(n) source during outgrowth.
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k this deck
42
A phototrophic bacterium swims outside the illuminated field of view of the microscope, reverses direction, and scurries back into the lighted area. This is an example of .
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k this deck
43
The lipid portion of the lipopolysaccharide (LPS) is known as lipid A.
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44
An individual rod- shaped bacterium is also called a(n) morphology, while two or more of these are collectively described as _ _. Similarly, one is a spherical bacterium, and two or more are termed .
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45
As a general statement, inclusions function as energy reserves or as a reservoir of structural building blocks.
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46
A purple bacterial cell is interpreted as gram- positive when it forms insoluble crystal violet- iodine complexes within the cell during the Gram stain.
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47
Lysozyme is an enzyme that can ultimately lyse and kill bacterial cells by breaking
fi- 1,4- glycosidic bonds in peptidoglycan.
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k this deck
48
In general, swimming is performed with flagella, whereas gliding uses other cellular components such as pili.
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k this deck
49
Porins are channels in the outer membranes of gram- positive Bacteria.
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50
In general, lipids in the archaeal membrane lack true fatty acids.
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51
The proton motive force helps drive cellular functions that require energy by maintaining a positively charged side of the cell and the other negatively charged.
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k this deck
52
Eukaryotes have in their cytoplasmic membranes, which serve to strengthen and stabilize the membrane and make it less flexible. Many Bacteria have similar molecules, known as _ , in their membranes that likely have a similar role.
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53
Some membrane proteins are involved in bioenergetic reactions, while others are involved in membrane transport.
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54
Smaller prokaryotic cells generally grow faster than larger ones due to a higher surface- area- to- volume ratio.
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55
Hopanoids strengthen the plasma membrane in prokaryotes and form a highly rigid membrane, analogous to the function of cholesterol in animals.
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56
The rigid layer that is present in the cell walls of Bacteria that is primarily responsible for the strength of the wall is known as .
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57
Pathogenic bacteria that contain S- layers are protected against host defense mechanisms.
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58
Despite the invariance of the peptidoglycan backbone's structure, there are more than 100 different types of peptidoglycan.
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59
Teichoic acids are commonly found in gram- negative cell walls.
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60
The peptide interbridge crosslinking is found ONLY in the cell walls of Bacteria.
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61
In phototaxis, photosynthetic organisms move away from a light source.
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62
Elaborate on why the discovery of endospores was important to microbiology.
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63
Describe the processes of endospore formation and germination.
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64
What is the evolutionary advantage of the monolayer often found in archaeal membranes?
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65
Chemotaxis is a sensory response affecting flagellar function.
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66
Describe the growth of a flagellum.
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67
Explain what would happen to a motile bacterium undergoing chemotaxis if the Fli proteins suddenly ceased to function.
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68
Organisms may respond to temporal rather than spatial gradients.
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69
Explain the differences between uniporters, symporters, and antiporters.
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70
Compare and contrast fimbriae and pili.
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71
Compare and contrast a capsule and a slime layer in terms of composition, organization, and function.
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72
Photoreceptors are analogous to chemoreceptors in that they are both proteinaceous sensors.
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73
Explain why prokaryotes tend to adapt more rapidly to changing environmental conditions and more easily exploit new habitats than do eukaryotes.
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74
Compare and contrast the location and activities of periplasm- binding proteins of ABC transport systems in gram- negative and gram- positive Bacteria.
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75
Compare and contrast aerotaxis and osmotaxis.
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76
Both hydrophilic and charged molecules readily diffuse through the cytoplasmic membrane.
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77
Speculate on why all prokaryotic transport systems require energy in some form or another.
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78
The existence of nanobacteria was confirmed in 2006.
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79
Poly- fi- hydroxybutyric acid (PHB) is a carbon- and energy- storing polymer.
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80
ABC transport systems are present in both gram- positive and gram- negative Bacteria.
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