Deck 20: DNA Tools and Biotechnology

Question 1

Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?

A)altering the nucleotide sequence of the DNA fragment
B)methylating the cytosine bases within the DNA fragment
C)increasing the length of the DNA fragment
D)decreasing the length of the DNA fragment
E)neutralizing the negative charges within the DNA fragment

Answer 1

A

Question 2

Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity?

A)gel electrophoresis
B)use of RNAi
C)in vitro mutagenesis
D)polymerase chain reaction
E)restriction fragment analysis

Answer 2

C

Question 3

Why is it so important to be able to amplify DNA fragments when studying genes?

A)DNA fragments are too small to use individually.
B)A gene may represent only a millionth of the cell's DNA.
C)Restriction enzymes cut DNA into fragments that are too small.
D)A clone requires multiple copies of each gene per clone.
E)It is important to have multiple copies of DNA in the case of laboratory error.

Answer 3

B

Question 4

In order to identify a specific restriction fragment using a probe,what must be done?

A)The fragments must be separated by electrophoresis.
B)The fragments must be treated with heat or chemicals to separate the strands of the double helix.
C)The probe must be hybridized with the fragment.
D)The fragments must be separated by electrophoresis and the fragments must be treated with heat or chemicals to separate the strands of the double helix.
E)The fragments must be separated by electrophoresis, the fragments must be treated with heat or chemicals to separate the strands of the double helix, and the probe must be hybridized with the fragment.

Question 5

The reason for using Taq polymerase for PCR is that

A)it is heat stable and can withstand the temperature changes of the cycler.
B)only minute amounts are needed for each cycle of PCR.
C)it binds more readily than other polymerases to primer.
D)it has regions that are complementary to primers.
E)All of these are correct.

Question 6

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I)Transform bacteria with a recombinant DNA molecule.
II)Cut the plasmid DNA using restriction enzymes.
III)Extract plasmid DNA from bacterial cells.
IV)Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V)Use ligase to seal plasmid DNA to nonplasmid DNA.

A)I, II, IV, III, V
B)II, III, V, IV, I
C)III, II, IV, V, I
D)III, IV, V, I, II
E)IV, V, I, II, III

Question 7

Which of the following is used to make complementary DNA (cDNA)from RNA?

A)restriction enzymes
B)gene cloning
C)DNA ligase
D)gel electrophoresis
E)reverse transcriptase

Question 8

Pax-6 is a gene that is involved in eye formation in many invertebrates,such as Drosophila.Pax-6 is found as well in vertebrates.A Pax-6 gene from a mouse can be expressed in a fly and the protein (PAX-6)leads to a compound fly eye.This information suggests which of the following?

A)Pax-6 genes are identical in nucleotide sequence.
B)PAX-6 proteins have identical amino acid sequences.
C)Pax-6 is highly conserved and shows shared evolutionary ancestry.
D)PAX-6 proteins are different for formation of different kinds of eyes.
E)PAX-6 from a mouse can function in a fly, but a fly's Pax-6 gene cannot function in a mouse.

Question 9

A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell.In order to have the mutated sequence disable the function of the gene,what must then occur?

A)recombination resulting in replacement of the wild type with the mutated gene
B)use of a microarray to verify continued expression of the original gene
C)replication of the cloned gene using a bacterial plasmid
D)transcription of the cloned gene using cDNA
E)attachment of the mutated gene to an existing mRNA to be translated

Question 10

What is the most important reason to sequence an entire genome of a model organism,such as that of C.elegans,a nematode?

A)it allows researchers to use the sequence to build a "better" nematode, which is resistant to disease.
B)it allows research on a group of organisms we do not usually care much about.
C)the nematode is a good animal model for trying out cures for viral illness.
D)a sequence that is found to have a particular function in the nematode is likely to have a closely related function in vertebrates.
E)a sequence that is found to have no introns in the nematode genome is likely to have acquired the introns from higher organisms.

Question 11

Why might a laboratory be using dideoxynucleotides?

A)to separate DNA fragments
B)to clone the breakpoints of cut DNA
C)to produce cDNA from mRNA
D)to sequence a DNA fragment
E)to visualize DNA expression

Question 12

How does a bacterial cell protect its own DNA from restriction enzymes?

A)by adding methyl groups to adenines and cytosines
B)by using DNA ligase to seal the bacterial DNA into a closed circle
C)by adding histones to protect the double-stranded DNA
D)by forming "sticky ends" of bacterial DNA to prevent the enzyme from attaching
E)by reinforcing the bacterial DNA structure with covalent phosphodiester bonds

Question 13

A gene that contains introns can be made shorter (but remain functional)for genetic engineering purposes by using

A)RNA polymerase to transcribe the gene.
B)a restriction enzyme to cut the gene into shorter pieces.
C)reverse transcriptase to reconstruct the gene from its mRNA.
D)DNA polymerase to reconstruct the gene from its polypeptide product.
E)DNA ligase to put together fragments of the DNA that code for a particular polypeptide.

Question 14

To introduce a particular piece of DNA into an animal cell,such as that of a mouse,you would find more probable success with which of the following methods?

A)the shotgun approach
B)electroporation followed by recombination
C)introducing a plasmid into the cell
D)infecting the mouse cell with a Ti plasmid
E)transcription and translation

Question 15

Why are yeast cells frequently used as hosts for cloning?

A)They easily form colonies.
B)They can remove exons from mRNA.
C)They do not have plasmids.
D)They are eukaryotic cells.
E)Only yeast cells allow the gene to be cloned.

Question 16

Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
1)The primers hybridize to the target DNA.
2)The mixture is heated to a high temperature to denature the double-stranded target DNA.
3)Fresh DNA polymerase is added.
4)DNA polymerase extends the primers to make a copy of the target DNA.

A)2, 1, 4
B)1, 3, 2, 4
C)3, 4, 1, 2
D)3, 4, 2
E)2, 3, 4

Question 17

A principal problem with inserting an unmodified mammalian gene into a plasmid,and then getting that gene expressed in bacteria,is that

A)prokaryotes use a different genetic code from that of eukaryotes.
B)bacteria translate polycistronic messages only.
C)bacteria cannot remove eukaryotic introns.
D)bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
E)bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.

Question 18

DNA microarrays have made a huge impact on genomic studies because they

A)can be used to eliminate the function of any gene in the genome.
B)can be used to introduce entire genomes into bacterial cells.
C)allow the expression of many or even all of the genes in the genome to be compared at once.
D)allow physical maps of the genome to be assembled in a very short time.
E)dramatically enhance the efficiency of restriction enzymes.

Question 19

Assume that you are trying to insert a gene into a plasmid.Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X.The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y.You have a plasmid with a single site for Y,but not for X.Your strategy should be to

A)insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.
B)cut the plasmid with restriction enzyme X and insert the fragments cut with restriction enzyme Y into the plasmid.
C)cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
D)cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
E)cut the plasmid with restriction enzyme X and then insert the gene into the plasmid.

Question 20

For which of the following might RNAi more possibly be useful?

A)to raise the rate of production of a needed digestive enzyme
B)to decrease the production from a harmful gain-of-function mutated gene
C)to destroy an unwanted allele in a homozygous individual
D)to form a knockout organism that will not pass the deleted sequence to its progeny
E)to raise the concentration of a desired protein

Question 21

As genetic technology makes testing for a wide variety of genotypes possible,which of the following is likely to be an increasingly troublesome issue?

A)use of genotype information to provide positive identification of criminals
B)using technology to identify genes that cause criminal behaviours
C)the need to legislate for the protection of the privacy of genetic information
D)discrimination against certain racial groups because of major genetic differences
E)alteration of human phenotypes to prevent early disease

Question 22

Which of the following is true of embryonic stem cells but not of adult stem cells?

A)They can differentiate into many cell types.
B)They make up the majority of cells of the tissue from which they are derived.
C)They can continue to replicate for an indefinite period.
D)They can provide enormous amounts of information about the process of gene regulation.
E)One aim of using them is to provide cells for repair of diseased tissue.

Question 23

Which of the following is most closely identical to the formation of twins?

A)cell cloning
B)therapeutic cloning
C)use of adult stem cells
D)embryo transfer
E)organismal cloning

Question 24

Reproductive cloning of human embryos is generally considered unethical.However,on the subject of therapeutic cloning there is a wider divergence of opinion.Which of the following is a likely explanation?

A)Use of adult stem cells is likely to produce more cell types than use of embryonic stem cells.
B)Cloning to produce embryonic stem cells may lead to great medical benefits for many.
C)Cloning to produce stem cells relies on a different initial procedure than reproductive cloning.
D)A clone that lives until the blastocyst stage does not yet have human DNA.
E)No embryos would be destroyed in the process of therapeutic cloning.

Question 25

Genetic engineering is being used by the pharmaceutical industry.Which of the following is not currently one of the uses?

A)production of human insulin
B)production of human growth hormone
C)production of tissue plasminogen activator
D)genetic modification of plants to produce vaccines
E)creation of products that will remove poisons from the human body

Question 26

Which of the following problems with animal cloning might result in premature death of the clones?

A)use of pluripotent instead of totipotent stem cells
B)use of nuclear DNA as well as mtDNA
C)abnormal regulation due to variant methylation
D)the indefinite replication of totipotent stem cells
E)abnormal immune function due to bone marrow dysfunction

Question 27

Scientists developed a set of guidelines to address the safety of DNA technology.Which of the following is one of the adopted safety measures?

A)Microorganisms used in recombinant DNA experiments are genetically crippled to ensure that they cannot survive outside of the laboratory.
B)Genetically modified organisms are not allowed to be part of our food supply.
C)Transgenic plants are engineered so that the plant genes cannot hybridize.
D)Experiments involving HIV or other potentially dangerous viruses have been banned.
E)Recombinant plasmids cannot be replicated.

Question 28

For a particular microarray assay (DNA chip),cDNA has been made from the mRNAs of a dozen patients' breast tumour biopsies.The researchers will be looking for

A)a particular gene that is amplified in all or most of the patient samples.
B)a pattern of fluorescence that indicates which cells are overproliferating.
C)a pattern shared among some or all of the samples that indicates gene expression differing from control samples.
D)a group of cDNAs that act differently from those on the rest of the grid.
E)a group of cDNAs that match those in non-breast cancer control samples from the same population.

Question 29

Which of the following questions would not be answered using RNAi?

A)What is the function of gene 432 in this species of annelid?
B)What will happen in this insect's digestion if gene 173 is not able to be translated?
C)Is gene HA292 responsible for this disorder in humans?
D)Will the disabling of this gene in Drosophila and in a mouse cause similar results?
E)Is the gene on Drosophila chromosome 2L at this locus responsible for part of its production of nitrogen waste?

Question 30

In animals,what is the difference between reproductive cloning and therapeutic cloning?

A)Reproductive cloning uses totipotent cells, whereas therapeutic cloning does not.
B)Reproductive cloning uses embryonic stem cells, whereas therapeutic cloning does not.
C)Therapeutic cloning uses nuclei of adult cells transplanted into enucleated nonfertilized eggs.
D)Therapeutic cloning supplies cells for repair of diseased or injured organs.
E)Reproductive cloning results in the production of multiple egg or sperm cells.

Question 31

In 1997,Dolly the sheep was cloned.Which of the following processes was used?

A)use of mitochondrial DNA from adult female cells of another ewe
B)replication and dedifferentiation of adult stem cells from sheep bone marrow
C)separation of an early stage sheep blastula into separate cells, one of which was incubated in a surrogate ewe
D)fusion of an adult cell's nucleus with an enucleated sheep egg, followed by incubation in a surrogate
E)isolation of stem cells from a lamb embryo and production of a zygote equivalent

Question 32

Genetically engineered plants

A)are more difficult to engineer than animals.
B)include a transgenic rice plant that can grow in water three times as salty as seawater.
C)are being rapidly developed, but traditional plant breeding programs are still the only method used to develop new plants.
D)are able to fix nitrogen themselves.
E)are banned throughout the world.

Question 33

One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA)to bone marrow cells of a child with severe combined immunodeficiency (SCID),and delivery of these engineered cells back to the bone marrow of the affected child.What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?

A)The engineered bone marrow cells from this patient can be used for any other SCID patient.
B)The ADA-introduced allele causes all other ADA-negative cells to die.
C)The engineered cells, when reintroduced into the patient, find their way back to the bone marrow.
D)No vector is required to introduce the allele into ADA-negative cells.
E)The immune system fails to recognize cells with the variant gene.

Question 34

The first cloned cat,called Carbon Copy,was a calico,but she looked significantly different from her female parent.Why?

A)The environment, as well as genetics, affects phenotypic variation.
B)Fur-colour genes in cats are influenced by differential acetylation patterns.
C)Cloned animals have been found to have a higher frequency of transposon activation.
D)X inactivation in the embryo is random and produces different patterns.
E)The telomeres of the parent's chromosomes were shorter than those of an embryo.

Question 35

Which of the following is one of the technical reasons why gene therapy is problematic?

A)Most cells with an engineered gene do not produce gene product.
B)Most cells with engineered genes overwhelm other cells in a tissue.
C)Cells with transferred genes are unlikely to replicate.
D)Transferred genes may not have appropriately controlled activity.
E)mRNA from transferred genes cannot be translated.

Question 36

Which of the following statements is (are)correct about restriction enzymes?
I)They are used by bacteria to cut up foreign DNA.
II)They cut DNA unevenly to produce "sticky ends."
III)They are non-specific and cut anywhere along a sequence.
IV)They are involved with heterochromatin production.

A)I only
B)IV only
C)I and II
D)I, II, and III
E)III and IV

Question 37

A researcher is using adult stem cells and comparing them to other adult cells from the same tissue.Which of the following is a likely finding?

A)The cells from the two sources exhibit different patterns of DNA methylation.
B)Adult stem cells have more DNA nucleotides than their counterparts.
C)The two kinds of cells have virtually identical gene expression patterns in microarrays.
D)The nonstem cells have fewer repressed genes.
E)The nonstem cells have lost the promoters for more genes.

Question 38

Let us suppose that someone is successful at producing induced pluripotent stem cells (iPS)for replacement of pancreatic insulin-producing cells for people with type 1 diabetes.Which of the following could still be problems?
I)the possibility that,once introduced into the patient,the iPS cells produce nonpancreatic cells
II)the failure of the iPS cells to take up residence in the pancreas
III)the inability of the iPS cells to respond to appropriate regulatory signals

A)I only
B)II only
C)III only
D)I and II
E)I, II, and III

Question 39

In recent times,it has been shown that adult cells can be induced to become pluripotent stem cells (iPS).In order to make this conversion,what has been done to the adult cells?

A)A retrovirus is used to introduce four specific regulatory genes.
B)The adult stem cells must be fused with embryonic cells.
C)Cytoplasm from embryonic cells is injected into the adult cells.
D)An adenovirus vector is used to transfer embryonic gene products into adult cells.
E)The nucleus of an embryonic cell is used to replace the nucleus of an adult cell.

Question 40

In large-scale,genome-wide association studies in humans using SNPs,correlation is sought between

A)lengthy sequences that might be shared by most members of a population.
B)single nucleotide polymorphisms found only in persons with a particular disorder.
C)single nucleotide polymorphisms found in families with a particular intron sequence.
D)single nucleotide polymorphisms in two or more adjacent genes.
E)large inversions that displace the centromere.

Question 41

Use the following figure to answer the questions below.The DNA profiles that follow represent four different individuals.

Which of the following statements is most consistent with the results?

A)B is the child of A and C.
B)C is the child of A and B.
C)D is the child of B and C.
D)A is the child of B and C.
E)A is the child of C and D.

Question 42

Modern forensic investigation uses ________ to identify criminals.

A)cloning
B)RT-PCR
C)restriction fragment length polymorphism
D)single nucleotide polymorphism
E)short tandem repeats

Question 43

In animals,embryonic stem cells differ from adult stem cells in that

A)embryonic stem cells are totipotent, and adult stem cells are pluripotent.
B)embryonic stem cells are pluripotent, and adult stem cells are totipotent.
C)embryonic stem cells have more genes than adult stem cells.
D)embryonic stem cells have fewer genes than adult stem cells.
E)embryonic stem cells are localized to specific sites within the embryo, whereas adult stem cells are spread throughout the body.

Question 44

Use the following figure to answer the questions below.The DNA profiles that follow represent four different individuals.

Which of the following statements is most likely true?

A)D is the child of A and C.
B)D is the child of A and B.
C)D is the child of B and C.
D)A is the child of C and D.
E)B is the child of A and C.

Question 45

For applications in gene therapy,what is the most favorable characteristic of retroviruses?

A)Retroviruses have an RNA genome.
B)Retroviruses possess reverse transcriptase.
C)DNA copies of retroviral genomes become integrated into the genome of the infected cell.
D)Retroviruses mutate often.
E)Retroviruses are not immunogenic.

Question 46

How difficult is it to clone a plant?

A)very difficult, since retroviruses are needed
B)easy, as even mature differentiated cells are totipotent
C)easy, as even mature differentiated cells a pluripotent
D)impossible, since restriction enzymes prevent gene duplication
E)it depends entirely on the age of the plant

Question 47

Use the following figure to answer the questions below.The DNA profiles that follow represent four different individuals.

A linear piece of viral DNA of 8000 base pairs in length (8kb)can be cut with either of two restriction enzymes (X or Y).These are subjected to electrophoresis and produce the following bands:

Cutting the same 8 kb piece with both enzymes together results in bands at 4.0,2.5,1.0,and 0.5.
Of the possible arrangements of the sites given below,which one is most likely?

A)
B)
C)
D)
E)

Question 48

Use the following information to answer the next few questions.
A group of six students has taken samples of their own cheek cells,purified the DNA,and used a restriction enzyme known to cut at zero,one,or two sites in a particular gene of interest.
Analysis of the data obtained shows that two students each have two fragments,two students each have three fragments,and two students each have one only.What does this demonstrate?

A)Each pair of students has a different gene for this function.
B)The two students who have two fragments have one restriction site in this region.
C)The two students who have two fragments have two restriction sites within this gene.
D)The students with three fragments are said to have "fragile sites."
E)Each of these students is heterozygous for this gene.

Question 49

Why might using retroviral vectors for gene therapy increase the patient's risk of developing cancer?

A)Retroviral vectors might introduce proteins from the virus.
B)Retroviral vectors might not express the genes that were introduced into a patient's cells.
C)Retroviral vectors might not integrate their recombinant DNA into the patient's genome.
D)Retroviral vectors might integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integration.
E)Retroviral vectors might cause a strong immune response.

Question 50

An unspecialized cell that can reproduce and differentiate is a(n)________ cell.

A)pluripotent
B)tumour
C)zygote
D)stem
E)mutant

Question 51

The segment of DNA shown in the figure above has restriction sites I and II,which create restriction fragments A,B,and C.Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

A)
B)
C)
D)
E)

Question 52

An animal that has genes from another species is said to be

A)a mutant.
B)transgenic.
C)transposed.
D)synthetic.
E)an abomination.

Question 53

A cell of a particular type,that has the ability to differentiate into other cells of the same tissue type,is a(n)________ cell.

A)totipotent
B)pluripotent
C)tumour
D)metastatic
E)zygote

Question 54

Use the following information to answer the next few questions.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow in

A)the nutrient broth only.
B)the nutrient broth and the tetracycline broth only.
C)the nutrient broth, the ampicillin broth, and the tetracycline broth.
D)all four types of broth.
E)the ampicillin broth and the nutrient broth.

Question 55

Use the following information to answer the next few questions.
A group of six students has taken samples of their own cheek cells,purified the DNA,and used a restriction enzyme known to cut at zero,one,or two sites in a particular gene of interest.
Why might they be conducting such an experiment?

A)to find the location of this gene in the human genome
B)to prepare to isolate the chromosome on which the gene of interest is found
C)to find which of the students has which alleles
D)to collect population data that can be used to assess natural selection
E)to collect population data that can be used to study genetic drift

Question 56

Use the following figure to answer the questions below.The DNA profiles that follow represent four different individuals.

Which of the following are probably siblings?

A)A and B
B)A and C
C)A and D
D)C and D
E)B and D

Question 57

Which enzyme was used to produce the molecule in the figure above?

A)ligase
B)transcriptase
C)a restriction enzyme
D)RNA polymerase
E)DNA polymerase

Question 58

Use the following information to answer the next few questions.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that do not take up any plasmids would grow on which media?

A)the nutrient broth only
B)the nutrient broth and the tetracycline broth
C)the nutrient broth and the ampicillin broth
D)the tetracycline broth and the ampicillin broth
E)all three broths

Question 59

Use the following information to answer the next few questions.
A group of six students has taken samples of their own cheek cells,purified the DNA,and used a restriction enzyme known to cut at zero,one,or two sites in a particular gene of interest.
Their next two steps,in order,should be

A)use of a fluorescent probe for the gene sequence, then electrophoresis.
B)electrophoresis of the fragments followed by autoradiography.
C)electrophoresis of the fragments, followed by the use of a probe.
D)use of a ligase that will anneal the pieces, followed by PCR.
E)use of reverse transcriptase to make cDNA, followed by electrophoresis.

Question 60

Use the following information to answer the next few questions.
A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that contain the plasmid,but not the eukaryotic gene,would grow

A)in the nutrient broth plus ampicillin, but not in the broth containing tetracycline.
B)only in the broth containing both antibiotics.
C)in the broth containing tetracycline, but not in the broth containing ampicillin.
D)in all four types of broth.
E)in the nutrient broth without antibiotics only.

Question 61

Breast tumour biopsy specimens can be typed for a number of gene expression patterns.Together,these can provide risk analysis for the likely aggressive growth and metastasis of the tumour.How can this most help the physician and patient?

A)Some patients want to know as much as possible.
B)This can help them to decide whether and what kind of chemotherapy is warranted.
C)This can help them decide whether the tumour should be removed.
D)Some physicians may use the information to decide what to do, but not tell the patient.
E)This can help to aggregate health statistics.

Question 62

What characteristic of short tandem repeats (STRs)makes them useful for DNA fingerprinting?

A)The number of repeats varies widely from person to person or animal to animal.
B)The sequence of DNA that is repeated varies significantly from individual to individual.
C)The sequence variation is acted upon differently by natural selection in different environments.
D)Every racial and ethnic group has inherited different short tandem repeats.
E)Each STR can be correlated with a specific disease.

Question 63

Which of the following uses labeled probes to visualize the expression of genes in whole tissues and organisms?

A)RT-PCR
B)in situ hybridization
C)DNA microarrays
D)RNA interference
E)next generation sequencing

Question 64

The final step in a Sanger DNA sequencing reaction is to run the DNA fragments on a gel.What is the purpose of running DNA fragments on a gel?

A)It adds ddNTP to the end of each DNA fragment.
B)It changes the length of the DNA fragments.
C)It separates the DNA fragments based on their charge.
D)It separates the DNA fragments generated based on their size.
E)It adds dNTPs to the end of each DNA fragment.

Question 65

In recombinant DNA methods,the term vector can refer to

A)the enzyme that cuts DNA into restriction fragments.
B)the sticky end of a DNA fragment.
C)a SNP marker.
D)a plasmid used to transfer DNA into a living cell.

Question 66

Use the following information to answer the questions below.
Spider silk is one of the strongest biomolecules known.It has a tensile strength greater than Kevlar and elasticity greater than rubber.Attempts to "farm" spiders have met with little success (they tend to eat each other).Researchers have identified the gene responsible for the formation of the silk.
Plants are more readily manipulated by genetic engineering than are animals because

A)plant genes do not contain introns.
B)more vectors are available for transferring recombinant DNA into plant cells.
C)a somatic plant cell can often give rise to a complete plant.
D)plant cells have larger nuclei.
E)plants are easier to infect with virus than other eukaryotic organisms.

Question 67

DNA technology has many medical applications.Which of the following is not done routinely at present?

A)production of hormones for treating diabetes and dwarfism
B)production of microbes that can metabolize toxins
C)introduction of genetically engineered genes into human gametes
D)prenatal identification of genetic disease alleles

Question 68

Imagine that you compare two DNA sequences found in the same location on homologous chromosomes.On one of the homologs,the sequence is 5'AACTACGA3'.On the other homolog,the sequence is 5'AACTTCGA3'.Within a population,you discover that each of these sequences is common.Which of the following is most likely true about these sequences?

A)They contain an SNP that may be useful for genetic mapping.
B)They identify a protein-coding region of a gene.
C)They cause disease.
D)They are part of a promoter sequence.
E)They may be important for differentiation.

Question 69

Which of the following is in the correct order for one cycle of polymerase chain reaction (PCR)?

A)Denature DNA; add fresh enzyme; anneal primers; add dNTPs; extend primers.
B)Anneal primers; denature DNA; extend primers.
C)Extend primers; anneal primers; denature DNA.
D)Denature DNA; anneal primers; extend primers.
E)Denature DNA; add fresh enzyme; add dNTPs; anneal primers; extend primers.

Question 70

Use the following information to answer the questions below.
Spider silk is one of the strongest biomolecules known.It has a tensile strength greater than Kevlar and elasticity greater than rubber.Attempts to "farm" spiders have met with little success (they tend to eat each other).Researchers have identified the gene responsible for the formation of the silk.
What would be a plausible suggestion to produce this protein for human use?

A)Breed less aggressive spiders until they are "domesticated."
B)Identify the protein and synthesize it using other materials.
C)Insert the gene into another organism that is easier to culture and have it produce the silk protein.
D)Never mind-it's too difficult and too small a product to try.
E)There is nothing that can be done at this stage in our understanding but in time there may be a solution.

Question 71

Use the following information to answer the questions below.
Spider silk is one of the strongest biomolecules known.It has a tensile strength greater than Kevlar and elasticity greater than rubber.Attempts to "farm" spiders have met with little success (they tend to eat each other).Researchers have identified the gene responsible for the formation of the silk.
Researchers have successfully inserted this gene into goats.These "spidergoats" live a normal life.The silk protein is expressed and extracted from their milk.This is quite controversial; why not manufacture the silk from E.coli?

A)E) coli cannot be transformed.
B)Spiders are eukaryotic and their genes must be inserted into another eukaryote.
C)The silk protein would be mixed with other protein products in E. coli.
D)The sheer volume of protein extracted from milk makes it more economical.
E)Goats are more closely related to spiders than bacteria.

Question 72

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird).To compare a specific region of the DNA from the sample with DNA from living birds,which of the following would be most useful for increasing the amount of dodo DNA available for testing?

A)SNP analysis
B)polymerase chain reaction (PCR)
C)electroporation
D)gel electrophoresis

Question 73

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges.The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of

A)post-transcriptional processing.
B)post-translational processing.
C)nucleic acid hybridization.
D)restriction fragment ligation.

Question 74

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?

A)It could be amplified by the polymerase chain reaction.
B)It was produced from pre-mRNA using reverse transcriptase.
C)It could be labelled and used as a probe to detect genes expressed in the brain.
D)It lacks the introns of the pre-MRNA.

Question 75

Which of the following statements is correct when comparing two common sequencing techniques,the chain termination method and next generation sequencing?

A)The chain termination method is faster and more efficient, so it is used to generate large-scale sequences, while next generation synthesis is used for routine, small-scale jobs.
B)The chain termination method employs the polymerase chain reaction, but next generation sequencing does not.
C)In the chain termination method, the order of bases is detected by fluorescently labeling each dideoxynucleotide in a different color, while next generation sequencing determines the order of bases by detecting the release of PPi during the formation of the phosphodiester bond.
D)Next generation sequencing employs electrophoresis, but the chain termination method does not.
E)Next generation sequencing moves a single strand of DNA through a very long pore, but the chain termination method does not.

Question 76

One possible use of transgenic plants is in the production of human proteins,such as vaccines.Which of the following is a possible hindrance that must be overcome?

A)prevention of transmission of plant allergens to the vaccine recipients
B)prevention of vaccine-containing plants being consumed by insects
C)use of plant cells to translate non-plant-derived mRNA
D)inability of the human digestive system to accept plant-derived protein
E)the need to cook all such plants before consuming them

Question 77

What information is critical to the success of polymerase chain reaction (PCR)?

A)The DNA sequence of the ends of the DNA to be amplified must be known.
B)The complete DNA sequence of the DNA to be amplified must be known.
C)The sequence of restriction-enzyme recognition sites in the DNA to be amplified must be known.
D)The sequence of restriction-enzyme recognition sites in the DNA to be amplified and in the
Plasmid where the amplified DNA fragment will be cloned must be known.
E)The plasmid must have a low GC content.

Question 78

In an experiment,the bacteriophage Lambda genome was cut into fragments using the restriction enzyme Hind III.Hind III cuts the Lambda producing eight different size fragments when separated by gel electrophoresis.Select an observation that best describes a correct aspect of the two processes of restriction digest and gel electrophoresis:

A)When separated on a gel, the pattern of DNA bands will be characteristic of those cut with Hind III, different restriction enzymes will not produce these same fragments.
B)The Hind III sequence is found eight times in the Lambda genome.
C)If an electrical current is not used, eight separate DNA bands would be visible, but they would not be separated as much as when an electrical current is used.
D)Only the restriction enzyme Hind III can be used to cut Lambda DNA, since restriction enzymes are specific to the type of DNA they can cut.
E)Since the Lambda genome was cut by Hind III, the genome must be composed of RNA.