Deck 25: Biotechnology

Question 1

Which of the following is a vector created by the combination of viral DNA fragments and fragments from a plasmid?

A)Cosmid vector
B)Plasmid vector
C)Combined vector
D)Lambda phage vector

Answer 1

A

Question 2

A portion of DNA,such as a gene,that is synthesized from an RNA template is

A)recombinant DNA.
B)probe DNA.
C)complementary DNA.
D)reverse transcriptase.

Answer 2

C

Question 3

Enzymes that cut DNA straight across both of the double helix strands produce __________ ends.

Answer 3

blunt

Question 4

Reverse transcriptase is a(n)

A)RNA polymerase from the nucleus.
B)reactive RNA probe.
C)synthetic RNA polymerase.
D)DNA polymerase that uses RNA as a template.

Question 5

The recombinant protein that is used to help dissolve blood clots such as those that occur in heart attacks and stroke is

A)interferon.
B)factor VIII.
C)antisense molecule.
D)tissue plasminogen activator.

Question 6

All of the following are steps in the polymerase chain reaction except

A)extension.
B)translation.
C)priming.
D)denaturation.

Question 7

Recombinant DNA technology is also referred to as __________.

Question 8

Which of the following tools of genetic engineering is used to make multiple copies of DNA?

A)PCR
B)Gel electrophoresis
C)Reverse transcription
D)Recombinant DNA

Question 9

Which of the following is a true statement about recombinant DNA technology?

A)It is a single technique for genetic manipulation.
B)It involves the modification of an organism's genome.
C)It will replace biotechnology in the future.
D)It manipulates only the genotype.

Question 10

The term endonuclease refers to

A)gene libraries.
B)restriction enzymes.
C)plasmids.
D)cDNA.

Question 11

Which of the following is NOT an advantage of phage therapy?

A)Specificity of phages to target bacteria
B)Specificity of phages to target viruses
C)Replication of phages at the site of infection
D)No or little side effects

Question 12

The genetic recombination process that occurs when bacteria acquire fragments of DNA from the local environment and then express the encoded proteins is called

A)transcription.
B)transformation.
C)transduction.
D)ligation.

Question 13

Enzymes that can cleave DNA at a particular site are __________.

Question 14

A genetic recombination process that uses a cytoplasmic bridge between the donor and the recipient is called

A)transduction.
B)ligation.
C)conjugation.
D)extension.

Question 15

The term "biotechnology" was created in 1919 by

A)Robert Koch.
B)Karl Ereky.
C)Robert Bud.
D)Louis Pasteur.

Question 16

Small loops of DNA found in the cytoplasm of bacteria are

A)cDNA.
B)mRNA.
C)plasmids.
D)clones.

Question 17

All of the following codons can code for arginine except

A)CGU.
B)AGA.
C)GGC.
D)AGG.

Question 18

The synthetic interferon that is used in the treatment of multiple sclerosis is

A)interferon alfa-2b.
B)interferon beta-1a.
C)interferon alfa-2a.
D)interferon gamma-1b.

Question 19

In PCR,which of the following temperature ranges is used in the priming process?

A)90-95 °C
B)50-70 °C
C)60-80 °C
D)35-65 °C

Question 20

Which of the following scientists was instrumental in the discovery of plasmids?

A)Robert Koch
B)Louis Pasteur
C)Stanley Cohen
D)Willard Johannsen

Question 21

The process of copying a plate to identify a specific colony is called __________.

Question 22

Match the description below with the correct item from this list.
a.cDNA
b.Eukaryotic genes
c.Prokaryotic genes
d.Escherichia coli
e.Transformation
f.DNA ligase
g.Staphylococcus aureus
h.Hemophilia
Permanent combining of sticky ends

Question 23

Match the description below with the correct item from this list.
a.cDNA
b.Eukaryotic genes
c.Prokaryotic genes
d.Escherichia coli
e.Transformation
f.DNA ligase
g.Staphylococcus aureus
h.Hemophilia
Factor VIII

Question 24

Match the description below with the correct item from this list.
a.cDNA
b.Eukaryotic genes
c.Prokaryotic genes
d.Escherichia coli
e.Transformation
f.DNA ligase
g.Staphylococcus aureus
h.Hemophilia
A genetic recombination process

Question 25

Match the description below with the correct item from this list.
a.cDNA
b.Eukaryotic genes
c.Prokaryotic genes
d.Escherichia coli
e.Transformation
f.DNA ligase
g.Staphylococcus aureus
h.Hemophilia
Introns

Question 26

Match the description below with the correct item from this list.
a.cDNA
b.Eukaryotic genes
c.Prokaryotic genes
d.Escherichia coli
e.Transformation
f.DNA ligase
g.Staphylococcus aureus
h.Hemophilia
The "workhorse" in genetic engineering

Question 27

The hormone produced by the kidney to stimulate stem cells of the bone marrow to mature into red blood cells is __________.

Question 28

The enzyme used to synthesize complementary DNA from an mRNA template is __________.

Question 29

When a single bacterium can produce millions of identical cells,the population is called a(n)__________.

Question 30

Genetically modified crops are also referred to as __________ plants.

Question 31

A sense strand is a __________ to __________ mRNA or DNA molecule.

Question 32

Synthetic proteins that can be used in the treatment of hepatitis C are __________.