Deck 7: Recombinant DNA Technology

Question 1

A collection of cloned DNA fragments is called a .

A)consortium
B)chromosomal database
C)chromosome library
D)gene library

Answer 1

D

Question 2

What types of genes are found on plasmids and often used as selectable markers?

A)antibiotic resistance genes
B)multiple cloning sites
C)origins of replication
D)methylated sequences

Answer 2

A

Question 3

Why must a shuttle vector possess two origins of replication instead of one?

A)Shuttle vectors must replicate in multiple hosts with each host requiring a different origin.
B)Shuttle vectors must be able to replicate from two different origins of replication depending upon the specific growth conditions.
C)Shuttle vectors only have one origin of replication,not two.
D)Shuttle vectors use one origin for replication and the other origin for shuttling recombinant DNA into new hosts.

Answer 3

A

Question 4

Yeast artificial chromosomes must have and in order to function within eukaryotes.

A)origin of replication;internal resolution sites
B)telomere sequences;centromere sequences
C)multiple cloning sites;genes for nuclear proteins
D)multiple promoters;genes for antibiotic resistance

Question 5

The enzyme that links the ends of two DNA molecules is called .

A)reverse transcriptase
B)DNA polymerase
C)DNA ligase
D)DNA helicase

Question 6

Which process enables recombinant DNA to be introduced into mammalian cell lines?

A)Transfection
B)Transduction
C)Transformation
D)Translocation

Question 7

Which of the following cloning vectors carries the largest insert relative to the others in the answer choices?

A)BACs
B)cosmids
C)PACs
D)plasmids

Question 8

Reverse transcriptase generates cDNA from a template.

A)DNA
B)transfer RNA
C)messenger RNA
D)cDNA

Question 9

The purpose of the centromere sequence in YACs is to .

A)provide a multiple cloning site
B)allow correct partitioning of the plasmid in yeast
C)allow the vector to replicate in the yeast
D)select for the presence of the vector in the host

Question 10

Restriction enzymes .

A)generate either blunt or sticky ends by cutting the DNA on each strand.
B)are nucleases.
C)recognize specific sequences and cleave the DNA at the recognition site or somewhere in the vicinity.
D)are all of the above.

Question 11

Cells methylate their own DNA to .

A)mark the DNA for degradation
B)prevent replication
C)protect the DNA from the cell's own restriction enzymes
D)stabilize the DNA double helix