Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 15

Let's suppose a cell line has become malignant because it has accumulated mutations that inactivate two different tumor-suppressor genes. A researcher followed the protocol described in the experiment of Figure 24.12 and isolated DNA from this mutant cell line. The DNA was used to transform normal fibroblast (NIH3T3) cells. What results would you expect Would you expect a high number of malignant foci or not Explain your answer.FIGURE 24.12 Identification of chromosomal oncogenes.
Starting material: Several mouse cell lines. Some of the cell lines were malignant due to exposure to chemical or physical mutagens, whereas others were normal. It was known that none of the cell lines in this experiment were infected with oncogenic viruses.