Molecular Biology 5th Edition by Robert Weaver

Exercise 1

You are studying a new class of eukaryotic promoters (class IV) recognized by a novel RNA polymerase IV. You discover two general transcription factors that are required for transcription from these promoters. Describe experiments you would perform to determine which, if any, is an assembly factor, and which is required to recruit the RNA polymerase to the promoter. Provide sample results of your experiments.