Molecular Biology 5th Edition by Robert Weaver

Exercise 4

You are investigating DNA replication in a new species of bacteria. You discover that this organism has a clamp and pol III*, similar to their counterparts in E. coli. You want to know whether this clamp and pol III* separate during idling and after termination on a model template. Describe the experiment you would use to answer this question. Include the assay for separation you would use, and present sample results.