Chat with AI
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 12
Quantitative relationships between rate constants to calculate K m , kinetic efficiency (k cat /K m ) and V max -IV
Carbonic anhydrase catalyzes the hydration of CO2 .
CO2 + H2 O ? H2 CO3
The K m of carbonic anhydrase for CO2 is 12 mM. Carbonic anhydrase gave an initial velocity v 0 = 4.5 µmols H2 CO3 formed/mL sec when [CO2 ] = 36 mM.
a. What is the V max for this enzyme?
b. Assuming 5 pmol/mL (5 x 10 -12 moles/mL) of enzyme were used in this experiment, what is k cat for this enzyme?
c. What is the catalytic efficiency of this enzyme?
d. Does carbonic anhydrase approach "catalytic perfection"?
Carbonic anhydrase catalyzes the hydration of CO2 .
CO2 + H2 O ? H2 CO3
The K m of carbonic anhydrase for CO2 is 12 mM. Carbonic anhydrase gave an initial velocity v 0 = 4.5 µmols H2 CO3 formed/mL sec when [CO2 ] = 36 mM.
a. What is the V max for this enzyme?
b. Assuming 5 pmol/mL (5 x 10 -12 moles/mL) of enzyme were used in this experiment, what is k cat for this enzyme?
c. What is the catalytic efficiency of this enzyme?
d. Does carbonic anhydrase approach "catalytic perfection"?
Explanation
Verified
Verified
a. The V max for the enzyme can be found...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Why don’t you like this exercise?
Other Minimum 8 character and maximum 255 character
Character 255
