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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 14
Quantitative relationships between rate constants to calculate K m , kinetic efficiency (k cat /K m ) and V max -VI
The enzyme catalase catalyzes the decomposition of hydrogen peroxide:
2 H2 O2 ? 2 H2 O + O2
The turnover number (k cat ) for catalase is 40,000,000 sec -1. The K m of catalase for its substrate H2 O2 is 0.11 M.
a. In an experiment using 3 nanomole/mL of catalase, what is V max ?
b. What is v when [H2 O2 ] is 0.75 M?
c. What is the catalytic efficiency of catalase?
d. Does catalase approach "catalytic perfection"?
The enzyme catalase catalyzes the decomposition of hydrogen peroxide:
2 H2 O2 ? 2 H2 O + O2
The turnover number (k cat ) for catalase is 40,000,000 sec -1. The K m of catalase for its substrate H2 O2 is 0.11 M.
a. In an experiment using 3 nanomole/mL of catalase, what is V max ?
b. What is v when [H2 O2 ] is 0.75 M?
c. What is the catalytic efficiency of catalase?
d. Does catalase approach "catalytic perfection"?
Explanation
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Verified
a. To find the V max use:
b. To find v...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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