Essential Genetics 6th Edition by Daniel Hartl

Exercise 1

The table below shows matching regions of the DNA, mRNA, tRNA, and amino acids encoded in a particular gene. The mRNA is shown with its 5' end at the left, and the tRNA anticodon is shown with its 3' end at the left. The vertical lines define the reading frame.
(a) Complete the nucleic acid sequences, assuming normal Watson-Crick pairing between each codon and anticodon.
(b) Is the DNA strand that is transcribed the top strand or the bottom strand?
(c) Translate the mRNA in all three reading frames.
(d) Specify the nucleic acid strand(s) whose sequence could be used as a probe in a Southern blot hybridization, in which the hybridization is carried out against genomic DNA.
(e) Specify the nucleic acid strand(s) whose sequence could be used as a probe in a Northern blot hybridization, in which the hybridization is carried out against mRNA.