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Essential Genetics 6th Edition by Daniel Hartl
Edition 6ISBN: 978-1449686482Essential Genetics 6th Edition by Daniel Hartl
Edition 6ISBN: 978-1449686482 Exercise 16
A kan-r tet-r plasmid is treated with the restriction enzyme Bgl I, which cleaves the kan (kanamycin) gene. The DNA is annealed with a Bgl I digest of Neurospora DNA and after ligation used to transform E. coli.
(a) What antibiotic would you put into the growth medium to ensure that each colony has the plasmid?
(b) What antibiotic-resistance phenotypes would be found among the resulting colonies?
(c) Which phenotype is expected to contain Neurospora DNA inserts?
(a) What antibiotic would you put into the growth medium to ensure that each colony has the plasmid?
(b) What antibiotic-resistance phenotypes would be found among the resulting colonies?
(c) Which phenotype is expected to contain Neurospora DNA inserts?
Explanation
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The plasmid, kan-r tet-r, when exposed t...
Essential Genetics 6th Edition by Daniel Hartl
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