Essential Genetics 6th Edition by Daniel Hartl

Exercise 15

Shown here is a restriction map of a 12-kb linear plasmid isolated from cells of Borrelia burgdorferi , a spirochete bacterium transmitted by the bite of Ixodes ticks that causes Lyme disease. The symbols D, P, C, H, K, S, and A represent cleavage sites for the restriction enzymes Dde I, Pst I, Cla I, Hind III, Kpn I, Sac I, and Alu I, respectively. In the accompanying gel diagram, show the positions at which bands would be found after digestion of the plasmid with the indicated restriction enzyme or enzymes.